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Synthetic peptide: YSPTSpPS (Human). The sequence is repeated multiple times in the C-terminal domain of RNA polymerase II.
Our Abpromise guarantee covers the use of ab5408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.|
|ChIP||Use 1-4µg for 106 cells.|
|ChIP/Chip||Use at an assay dependent dilution. PubMed: 19703992|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 260 kDa (predicted molecular weight: 217 kDa).|
|ELISA||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution. See Abreviews.|
|CHIPseq||Use 2-0.3 µg for µg of chromatin.|
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab5408 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
Lane 1: buffer,
Lane 2: extract from human 293T cells as positive control,
Lanes 3 and 4: egg extracts made from wild type N2 worms and worms grown on RNAi food against dicer,
Lanes 5-8: intact worms in mentioned larval stage and eggs boiled directly in SDS-PAGE buffer and loaded
This image was kindly supplied as part of the review submitted by Shveta Bagga.
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