Synthetic peptide corresponding to Human RNA polymerase II CTD repeat
YSPTSPS(C terminal) (phospho S2).
Our Abpromise guarantee covers the use of ab70324 in the following tested applications.
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2500 - 1/20000. Detects a band of approximately 270 kDa.|
|ELISA||Use at an assay dependent concentration.|
Serially diluted ab70324 was bound to immobilised phospho- or control peptides (1 microgram per mL). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 50000 times) and signal was developed by TMB substrate.
ab70324 has not yet been referenced specifically in any publications.