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A phosphorylated synthetic peptide, from human RNA Polymerase II from around serine 5 of the C-terminal repeated sequence YSPTSPS.
Our Abpromise guarantee covers the use of ab70158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||1/2500 - 1/20000. Detects a band of approximately 260 kDa (predicted molecular weight: 217 kDa).|
ab70158 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"