Anti-RNA Polymerase II p14.5 antibody (ab28436)

Overview

  • Product nameAnti-RNA Polymerase II p14.5 antibody
    See all RNA Polymerase II p14.5 primary antibodies
  • Description
    Rabbit polyclonal to RNA Polymerase II p14.5
  • Specificityab28436 recognises RNA polymerase II RPB9 subunit (p14.5).
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein p14.5 (Human)

  • Positive control
    • HeLa nuclear extract

Properties

Applications

Our Abpromise guarantee covers the use of ab28436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/2000 - 1/5000. Predicted molecular weight: 14.5 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template.
    • Sequence similaritiesBelongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.
      Contains 1 TFIIS-type zinc finger.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DNA directed RNA polymerase II 14.5 kDa polypeptide antibody
      • DNA directed RNA polymerase II polypeptide I antibody
      • DNA directed RNA polymerase II subunit I antibody
      • DNA directed RNA polymerase II subunit RPB9 antibody
      • DNA-directed RNA polymerase II subunit I antibody
      • DNA-directed RNA polymerase II subunit RPB9 antibody
      • hRPB14.5 antibody
      • POLR2I antibody
      • Polymerase (RNA) II (DNA directed) polypeptide I 14.5kDa antibody
      • RNA polymerase II 14.5 kDa subunit antibody
      • RNA polymerase II subunit B9 antibody
      • RPB14.5 antibody
      • RPB9 antibody
      • RPB9_HUMAN antibody
      see all

    References for Anti-RNA Polymerase II p14.5 antibody (ab28436)

    ab28436 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Caenorhabditis elegans Cell (intestinal nuclei)
    Specification intestinal nuclei
    Fixative Paraformaldehyde
    Permeabilization Yes - Sperm Salts Buffer, Triton X-100
    Username

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    Verified customer

    Submitted Jun 03 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"