RabMAb

Anti-RNA polymerase II RPB1 antibody [EPR1509Y] - ChIP Grade (ab76123)

Overview

  • Product nameAnti-RNA polymerase II RPB1 antibody [EPR1509Y] - ChIP Grade
    See all RNA polymerase II RPB1 primary antibodies
  • Description
    Rabbit monoclonal [EPR1509Y] to RNA polymerase II RPB1 - ChIP Grade
  • Tested applicationsWB, ICC, Flow Cyt, ChIPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N terminus of human RNA polymerase II

  • Positive control
    • HeLa cell lysate.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab76123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 270 kDa (predicted molecular weight: 217 kDa).
ICC 1/50.
Flow Cyt 1/100 - 1/1000.
ChIP Use at an assay dependent concentration. PubMed: 22249179
  • Application notesIs unsuitable for IHC-P or IP.
  • Target

    • RelevanceRPB1 is a subunit of RNA polymerase II which catalyses transcription of RNA from DNA. RPB1 forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template.
    • Cellular localizationNuclear
    • Database links
    • Alternative names
      • B220 antibody
      • DNA directed RNA polymerase II subunit RPB1 antibody
      • DNA-directed RNA polymerase II largest subunit antibody
      • hRPB220 antibody
      • hsRPB1 antibody
      • MGC75453 antibody
      • POLR2 antibody
      • POLR2A antibody
      • POLRA antibody
      • Polymerase (RNA) II (DNA directed) polypeptide A 220kDa antibody
      • polymerase (RNA) II (DNA directed) polypeptide A, 220kDa antibody
      • RNA polymerase II subunit 1 antibody
      • RNA polymerase II subunit B1 antibody
      • RP02 antibody
      • RPB220 antibody
      • RPBh1 antibody
      • RpIILS antibody
      • RPO21 antibody
      • RPOL2 antibody
      • SUA8 antibody
      see all

    Anti-RNA polymerase II RPB1 antibody [EPR1509Y] - ChIP Grade images

    • Overlay histogram showing HeLa cells stained with ab76123 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76123, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Anti-RNA polymerase II RPB1 antibody [EPR1509Y] - ChIP Grade (ab76123) at 1/2000 dilution + HeLa cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 217 kDa
      Observed band size : 270 kDa (why is the actual band size different from the predicted?)

    References for Anti-RNA polymerase II RPB1 antibody [EPR1509Y] - ChIP Grade (ab76123)

    This product has been referenced in:
    • Kim JW  et al. New molecular bridge between RelA/p65 and NF-?B target genes via histone acetyltransferase TIP60 cofactor. J Biol Chem 287:7780-91 (2012). ChIP ; Human . Read more (PubMed: 22249179) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"