Anti-RNA polymerase II RPB11 antibody (ab180719)

Overview

  • Product name
    Anti-RNA polymerase II RPB11 antibody
    See all RNA polymerase II RPB11 primary antibodies
  • Description
    Rabbit polyclonal to RNA polymerase II RPB11
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant full length protein corresponding to Human RNA polymerase II RPB11 aa 1-117.
    Sequence:

    MNAPPAFESF LLFEGEKKIT INKDTKVPNA CLFTINKEDH TLGNIIKSQL LKDPQVLFAG YKVPHPLEHK IIIRVQTTPD YSPQEAFTNA ITDLISELSL LEERFRVAIK DKQEGIE


    Database link: P52435

  • Positive control
    • K562, Raji and mouse thymus cell line extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab180719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/2000. Predicted molecular weight: 13 kDa.
IHC-P 1/50 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft.
  • Tissue specificity
    Ubiquitously expressed. High expression was found in heart and skeletal muscle.
  • Sequence similarities
    Belongs to the archaeal rpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA directed RNA polymerase II subunit RPB11 a antibody
    • DNA-directed RNA polymerase II 13.3 kDa polypeptide antibody
    • DNA-directed RNA polymerase II 13.3 kDa polypeptide antibody
    • DNA-directed RNA polymerase II subunit J 1 antibody
    • DNA-directed RNA polymerase II subunit J-1 antibody
    • DNA-directed RNA polymerase II subunit RPB11-a antibody
    • hRPB 14 antibody
    • hRPB14 antibody
    • POLR 2J1 antibody
    • POLR2J antibody
    • POLR2J1 antibody
    • polymerase (RNA) II (DNA directed) polypeptide J 13.3kDa antibody
    • polymerase (RNA) II (DNA directed) polypeptide J 13.3kDa antibody
    • RNA polymerase II 13.3 kDa subunit antibody
    • RNA polymerase II subunit B11 a antibody
    • RNA polymerase II subunit B11-a antibody
    • RPB 11 antibody
    • RPB 11A antibody
    • RPB 11m antibody
    • RPB11 antibody
    • RPB11_HUMAN antibody
    • RPB11a antibody
    • RPB11m antibody
    • Rpo2-4 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab180719. Blue DAPI for nuclear staining.
  • All lanes : Anti-RNA polymerase II RPB11 antibody (ab180719) at 1/500 dilution

    Lane 1 : K562 cell line extracts
    Lane 2 : Raji cell line extracts
    Lane 3 : mouse thymus cell line extracts

    Predicted band size: 13 kDa

References

ab180719 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab180719.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up