Anti-RNA polymerase II RPB4 antibody (ab57301)

Overview

  • Product nameAnti-RNA polymerase II RPB4 antibody
    See all RNA polymerase II RPB4 primary antibodies
  • Description
    Mouse monoclonal to RNA polymerase II RPB4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-143 of Human RNA polymerase II RPB4

Properties

Applications

Our Abpromise guarantee covers the use of ab57301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA.
    • Sequence similaritiesBelongs to the eukaryotic RPB4 RNA polymerase subunit family.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DNA directed RNA polymerase II 16 kDa polypeptide antibody
      • DNA directed RNA polymerase II subunit D antibody
      • DNA directed RNA polymerase II subunit RPB4 antibody
      • DNA-directed RNA polymerase II 16 kDa polypeptide antibody
      • DNA-directed RNA polymerase II subunit D antibody
      • DNA-directed RNA polymerase II subunit rpb4 antibody
      • HSRBP4 antibody
      • polr2d antibody
      • polymerase RNA II DNA directed polypeptide D antibody
      • RBP4 antibody
      • RNA polymerase II 16 kDa subunit antibody
      • RNA polymerase II subunit B4 antibody
      • RNA polymerase II subunit D antibody
      • RNA polymerase II subunit hsRBP4 antibody
      • RPB16 antibody
      • RPB4_HUMAN antibody
      see all

    Anti-RNA polymerase II RPB4 antibody images

    • Western blot against tagged recombinant protein immunogen using ab57301 RNA polymerase II RPB4 antibody at 1ug/ml. Predicted band size of immunogen is 41 kDa

    References for Anti-RNA polymerase II RPB4 antibody (ab57301)

    ab57301 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"