Overview

  • Product name
    Anti-RNase H1 antibody
  • Description
    Mouse monoclonal to RNase H1
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: AACKAIEQAK TQNINKLVLY TDSMFTINGI TNWVQGWKKN GWKTSAGKEV INKEDFVALE RLTQGMDIQW MHVPGHSGFI GNEEADRLAR EGAKQSED, corresponding to amino acids 189-287 of Human RNase H1

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab56560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 32 kDa.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    Rnase H1 specifically degrades the RNA of RNA-DNA hybrids.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • H1RNA antibody
    • MGC108918 antibody
    • Ribonuclease H type II antibody
    • RibonucleaseH1 antibody
    • RNaseH1 antibody
    • RNH1 antibody
    see all

Anti-RNase H1 antibody images

  • All lanes : Anti-RNase H1 antibody (ab56560) at 1 µg/ml

    Lane 1 : Human fibroblasts whole cell lysate - untransfected
    Lane 2 : Human fibroblasts whole cell lysate - transfected with RnaseH construct

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated sheep anti-mouse IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 59 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    The plasmid has a GFP tag which adds ~27kDa to the protein, expected size ~59kDa.

    See Abreview



  • Predicted band size : 32 kDa
    RNase H1 antibody (ab56560) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing Jurkat cells stained with ab56560 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56560, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-RNase H1 antibody (ab56560)

This product has been referenced in:
  • Jangi M  et al. SMN deficiency in severe models of spinal muscular atrophy causes widespread intron retention and DNA damage. Proc Natl Acad Sci U S A 114:E2347-E2356 (2017). WB ; Human . Read more (PubMed: 28270613) »
  • Akman G  et al. Pathological ribonuclease H1 causes R-loop depletion and aberrant DNA segregation in mitochondria. Proc Natl Acad Sci U S A 113:E4276-85 (2016). Read more (PubMed: 27402764) »

See all 3 Publications for this product

Product Wall

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (fibroblasts)
Specification
fibroblasts
Treatment
RnaseH over-expression, collected 48H post transfection
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Apr 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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