Predicted band size : 32 kDa RNase H1 antibody (ab56560) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Flow Cytometry-Anti-RNase H1 antibody(ab56560)
Overlay histogram showing Jurkat cells stained with ab56560 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56560, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-RNase H1 antibody (ab56560)
This product has been referenced in:
Akman G et al. Pathological ribonuclease H1 causes R-loop depletion and aberrant DNA segregation in mitochondria. Proc Natl Acad Sci U S A113:E4276-85 (2016).
Read more (PubMed: 27402764) »
Ruhanen H et al. Involvement of DNA ligase III and ribonuclease H1 in mitochondrial DNA replication in cultured human cells. Biochim Biophys Acta1813:2000-2007 (2011).
Read more (PubMed: 21878356) »