Overview

  • Product name
    Anti-RNase H1 antibody
  • Description
    Mouse monoclonal to RNase H1
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: AACKAIEQAK TQNINKLVLY TDSMFTINGI TNWVQGWKKN GWKTSAGKEV INKEDFVALE RLTQGMDIQW MHVPGHSGFI GNEEADRLAR EGAKQSED, corresponding to amino acids 189-287 of Human RNase H1

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab56560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 32 kDa.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    Rnase H1 specifically degrades the RNA of RNA-DNA hybrids.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • H1RNA antibody
    • MGC108918 antibody
    • Ribonuclease H type II antibody
    • RibonucleaseH1 antibody
    • RNaseH1 antibody
    • RNH1 antibody
    see all

Images

  • All lanes : Anti-RNase H1 antibody (ab56560) at 1 µg/ml

    Lane 1 : Human fibroblasts whole cell lysate - untransfected
    Lane 2 : Human fibroblasts whole cell lysate - transfected with RnaseH construct

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated sheep anti-mouse IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 59 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    The plasmid has a GFP tag which adds ~27kDa to the protein, expected size ~59kDa.

    See Abreview



  • Predicted band size : 32 kDa
    RNase H1 antibody (ab56560) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing Jurkat cells stained with ab56560 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56560, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Jangi M  et al. SMN deficiency in severe models of spinal muscular atrophy causes widespread intron retention and DNA damage. Proc Natl Acad Sci U S A 114:E2347-E2356 (2017). WB ; Human . Read more (PubMed: 28270613) »
  • Akman G  et al. Pathological ribonuclease H1 causes R-loop depletion and aberrant DNA segregation in mitochondria. Proc Natl Acad Sci U S A 113:E4276-85 (2016). Read more (PubMed: 27402764) »

See all 5 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (fibroblasts)
Specification
fibroblasts
Treatment
RnaseH over-expression, collected 48H post transfection
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Apr 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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