Anti-RNase H2 subunit C antibody (ab80880)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab80880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa).

Target

  • RelevanceThe RNase H2 complex is a heterotrimer composed of the catalytic subunit RNASEH2A and the non-catalytic subunits RNASEH2B and RNASEH2C (Ribonuclease H2 subunit C). Ribonuclease H2 subunit C is the non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. It participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Ribonuclease H2 subunit C also mediates the excision of single ribonucleotides from DNA:RNA duplexes.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • AGS3 antibody
    • Aicardi-Goutieres syndrome 3 protein antibody
    • AYP1 antibody
    • Ribonuclease H2 subunit C antibody
    • Ribonuclease HI subunit C antibody
    • RNase H1 small subunit antibody
    • RNASEH2C antibody
    • RNH2C antibody
    see all

Anti-RNase H2 subunit C antibody images

  • All lanes : Anti-RNase H2 subunit C antibody (ab80880) at 1 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 18 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

References for Anti-RNase H2 subunit C antibody (ab80880)

ab80880 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk McManus

Verified customer

Submitted Jun 16 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"