Anti-RNase H2 subunit C antibody (ab89726)

Overview

  • Product nameAnti-RNase H2 subunit C antibody
    See all RNase H2 subunit C primary antibodies
  • Description
    Rabbit polyclonal to RNase H2 subunit C
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human RNase H2 subunit C.

    (Peptide available as ab90250.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HEK293; HepG2. IHC-P: human colon adenocarcinoma FFPE tissue sections

Properties

Applications

Our Abpromise guarantee covers the use of ab89726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa).

Target

  • RelevanceThe RNase H2 complex is a heterotrimer composed of the catalytic subunit RNASEH2A and the non-catalytic subunits RNASEH2B and RNASEH2C (Ribonuclease H2 subunit C). Ribonuclease H2 subunit C is the non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. It participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Ribonuclease H2 subunit C also mediates the excision of single ribonucleotides from DNA:RNA duplexes.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • AGS3 antibody
    • Aicardi-Goutieres syndrome 3 protein antibody
    • AYP1 antibody
    • Ribonuclease H2 subunit C antibody
    • Ribonuclease HI subunit C antibody
    • RNase H1 small subunit antibody
    • RNASEH2C antibody
    • RNH2C antibody
    see all

Anti-RNase H2 subunit C antibody images

  • All lanes : Anti-RNase H2 subunit C antibody (ab89726) at 1 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 18 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
    Ribonuclease H2 subunit C contains a potential phosphorylation site (SwissProt) which may explain its migration at a higher molecular weight (20 kDa) than predicted (18 kDa).
  • IHC image of RNase H2 subunit C staining in human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89726, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • All lanes : Anti-RNase H2 subunit C antibody (ab89726) at 1 µg/ml

    Lane 1 : Mouse Pei1 whole cell lysate
    Lane 2 : Mouse Pei1 whole cell lysate
    Lane 3 : Mouse Pei1 whole cell lysate
    Lane 4 : Mouse Pei1 whole cell lysate
    Lane 5 : Mouse Pei1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 18 kDa
    Observed band size : 18 kDa


    Exposure time : 1 second

    This image is courtesy of an Abreview submitted by Sarah Deasy

    Knockdown of Rnaseh2c using shRNA along with scramble control.

    Lane 1: scramble shRNA
    Lane 2: shRNA #1
    Lane 3: shRNA #2
    Lane 4: shRNA #3
    Lane 5: shRNA #4

    See Abreview

References for Anti-RNase H2 subunit C antibody (ab89726)

ab89726 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Cell lysate - whole cell (Pei1)
Specification Pei1
Treatment Knockdown of Rnaseh2c using shRNA along with scramble control
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Miss. Sarah Deasy

Verified customer

Submitted May 30 2014

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB ...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk McManus

Verified customer

Submitted Jun 16 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"