Overview

  • Product name
  • Description
    Rabbit polyclonal to RNASEH2B
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen: VVDNVFPNCI LLLKLPGLEK LLHHVTEEKG NPEIDNKKYY KYSKEKTLKW LEKKVNQTVA ALKTNNVNVS SRVQSTAFFS GDQASTDKEE DYIRYAH, corresponding to amino acids 117-213 of Human RNASEH2B (Q5TBB1).

  • Positive control
    • Human stomach tissue U-251 MG cells

Properties

Applications

Our Abpromise guarantee covers the use of ab122619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.
WB Use at an assay dependent concentration. PubMed: 22383524

Target

  • Function
    Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.
  • Tissue specificity
    Widely expressed.
  • Involvement in disease
    Defects in RNASEH2B are a cause of Aicardi-Goutieres syndrome type 2 (AGS2) [MIM:610181]. A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood.
  • Sequence similarities
    Belongs to the RNase H2 subunit B family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGS2 antibody
    • Aicardi-Goutieres syndrome 2 protein antibody
    • Deleted in lymphocytic leukemia 8 antibody
    • DLEU8 antibody
    • FLJ11712 antibody
    • Ribonuclease H2 subunit B antibody
    • Ribonuclease HI subunit B antibody
    • RNase H2 subunit B antibody
    • Rnaseh2b antibody
    • RNH2B_HUMAN antibody
    see all

Images

  • ab122619 at 1/50 dilution, staining RNASEH2B in Formalin/PFA-fixed paraffin-embedded human stomach section by immunohistochemistry.
  • ab122619, at 4 µg/ml, staining human cell line U-251 MG by Immunofluorescence. Antibody staining is shown in green.

References

This product has been referenced in:
  • Kennedy EM  et al. Frequent Incorporation of Ribonucleotides during HIV-1 Reverse Transcription and Their Attenuated Repair in Macrophages. J Biol Chem 287:14280-8 (2012). WB . Read more (PubMed: 22383524) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (fibroblast)
Permeabilization
Yes - Block step contains 0.3% TritonX-100
Specification
fibroblast
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

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Submitted Jun 29 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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