Overview

  • Product nameAnti-RNF11 antibody
    See all RNF11 primary antibodies
  • Description
    Rabbit polyclonal to RNF11
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 201-250 (GLIQHLPKGG YDPGRDGSEK KIRECVICMM DFVYGDPIRF LPCMHIYHLD) of Rat RNF11 (XM_237327).

  • Positive control
    • Rat lung lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab123141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionEssential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of TNFAIP3 to RIPK1 after TNF stimulation. TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Recruits STAMBP to the E3 ubiquitin-ligase SMURF2 for ubiquitination, leading to its degradation by the 26S proteasome.
  • Tissue specificityExpressed at low levels in the lung, liver, kidney, pancreas, spleen, prostate, thymus, ovary, small intestine, colon, and peripheral blood lymphocytes, and, at intermediate levels, in the testis, heart, brain and placenta. Highest expression in the skeletal muscle. In the brain, expressed at different levels in several regions: high levels in the amygdala, moderate in the hippocampus and thalamus, low in the caudate and extremely low levels in the corpus callosum (at protein level). Restricted to neurons, enriched in somatodendritic compartments and excluded from white matter (at protein level). In substantia nigra, present in cell bodies and processes of dopaminergic and nondopaminergic cells (at protein level). In Parkinson disease, sequestered in Lewy bodies and neurites. Overexpressed in breast cancer cells, but not detected in the surrounding stroma and weakly, if at all, in normal breast epithelial cells (at protein level). Also expressed in several tumor cell lines.
  • Sequence similaritiesContains 1 RING-type zinc finger.
  • DomainThe WW-binding motif mediates interaction with NEDD4.
  • Post-translational
    modifications
    Ubiquitinated in the presence of SMURF2 and UBE2D1, as well as WWP1.
    Phosphorylation by PKB/AKT1 may accelerate degradation by the proteasome.
  • Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic, when unphosphorylated, and nuclear, when phosphorylated by PKB/AKT1.
  • Information by UniProt
  • Database links
  • Alternative names
    • CGI 123 antibody
    • RING finger protein 11 antibody
    • RNF11 antibody
    • RNF11_HUMAN antibody
    • Sid 1669 antibody
    • SID1669 antibody
    see all

Anti-RNF11 antibody images

  • Anti-RNF11 antibody (ab123141) at 1 µg/ml + Rat lung lysate at 10 µg
    Developed using the ECL technique

    Predicted band size : 32 kDa

References for Anti-RNF11 antibody (ab123141)

ab123141 has not yet been referenced specifically in any publications.

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