Overview

  • Product nameAnti-RNF11 antibody
    See all RNF11 primary antibodies
  • Description
    Mouse monoclonal to RNF11
  • Tested applicationsSuitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: EEQIRIAQRI GLIQHLPKGV YDPGRDGSEK KIRECVICMM DFVYGDPIRF LPCMHIYHLD CIDDWLMRSF TCPSCMEPVD AALLSSYETN , corresponding to amino acids 65-155 of Human RNF11

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human skeletal muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab57180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionEssential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of TNFAIP3 to RIPK1 after TNF stimulation. TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Recruits STAMBP to the E3 ubiquitin-ligase SMURF2 for ubiquitination, leading to its degradation by the 26S proteasome.
  • Tissue specificityExpressed at low levels in the lung, liver, kidney, pancreas, spleen, prostate, thymus, ovary, small intestine, colon, and peripheral blood lymphocytes, and, at intermediate levels, in the testis, heart, brain and placenta. Highest expression in the skeletal muscle. In the brain, expressed at different levels in several regions: high levels in the amygdala, moderate in the hippocampus and thalamus, low in the caudate and extremely low levels in the corpus callosum (at protein level). Restricted to neurons, enriched in somatodendritic compartments and excluded from white matter (at protein level). In substantia nigra, present in cell bodies and processes of dopaminergic and nondopaminergic cells (at protein level). In Parkinson disease, sequestered in Lewy bodies and neurites. Overexpressed in breast cancer cells, but not detected in the surrounding stroma and weakly, if at all, in normal breast epithelial cells (at protein level). Also expressed in several tumor cell lines.
  • Sequence similaritiesContains 1 RING-type zinc finger.
  • DomainThe WW-binding motif mediates interaction with NEDD4.
  • Post-translational
    modifications
    Ubiquitinated in the presence of SMURF2 and UBE2D1, as well as WWP1.
    Phosphorylation by PKB/AKT1 may accelerate degradation by the proteasome.
  • Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic, when unphosphorylated, and nuclear, when phosphorylated by PKB/AKT1.
  • Information by UniProt
  • Database links
  • Alternative names
    • CGI 123 antibody
    • RING finger protein 11 antibody
    • RNF11 antibody
    • RNF11_HUMAN antibody
    • Sid 1669 antibody
    • SID1669 antibody
    see all

Anti-RNF11 antibody images

  • IHC image of RNF11 staining in Human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab57180, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot against tagged recombinant protein immunogen using ab57180 RNF11 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa
  • Overlay histogram showing MCF-7 cells stained with ab57180 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57180, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-RNF11 antibody (ab57180)

ab57180 has not yet been referenced specifically in any publications.

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. This antibody has only been tested in WB against the recombinant fragment used as ...

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Unfortunately, chain testing discounts are not possible.

Which antibody are you planning to buy? ab57180 is tested and guaranteed to recognize the endogenous protein. Please see the flow cytometry image wit...

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DISCOUNT CODE: ***
Expiration date: July 5th, 2012

I am very pleased to hear you would like to accept our offer and test ab123140 in human. This code will give you 1 free primary antibody before the expiration date. To redeem this offer,...

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Thank you very much for your interest in our RNF11 antibodies.

To our knowledge,neither ab57180 or ab123140have been tested on endogenous human samples. While we donot offer free or trial sized samples of any of our products, I can offer a d...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"