Overview

  • Product nameAnti-RNF111 antibody
    See all RNF111 primary antibodies
  • Description
    Mouse monoclonal to RNF111
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-109 of Human RNF111

Properties

Applications

Our Abpromise guarantee covers the use of ab57416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActs in the NODAL pathway of mesoderm patterning during embryonic development. Acts downstream AXIN1 as an E3 ubiquitin-protein ligase which promotes the ubiquitination of inhibitory SMADs such as SMAD7, induces their proteasomal degradation and thereby enhances the transcriptional activity of TGF-beta and BMP. Activates Smad3/Smad4-dependent transcription by triggering signal-induced SnoN degradation.
    • Tissue specificityBroadly expressed.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesContains 1 RING-type zinc finger.
    • Cellular localizationNucleus. Cytoplasm. Upon TGF-beta treatment, translocates from nucleus to cytosol.
    • Information by UniProt
    • Database links
    • Alternative names
      • ARK antibody
      • Arkadia antibody
      • DKFZp313E0731 antibody
      • DKFZp686H1966 antibody
      • DKFZp761D081 antibody
      • E3 ubiquitin protein ligase Arkadia antibody
      • E3 ubiquitin-protein ligase Arkadia antibody
      • FLJ38008 antibody
      • RING finger protein 111 antibody
      • RN111_HUMAN antibody
      • RNF 111 antibody
      • RNF111 antibody
      see all

    Anti-RNF111 antibody images

    • Western blot against tagged recombinant protein immunogen using ab57416 RNF111 antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa

    References for Anti-RNF111 antibody (ab57416)

    ab57416 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (aortic smooth muscle cell)
    Loading amount 20 µg
    Specification aortic smooth muscle cell
    Gel Running Conditions Reduced Denaturing (3-8% Tris-Acetate)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

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    Verified customer

    Submitted Mar 12 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"