Overview

  • Product nameAnti-RNF111 antibody
    See all RNF111 primary antibodies
  • Description
    Mouse polyclonal to RNF111
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length Human RNF111 (AAH60862.1).

  • Positive control
    • Rat brain tissue lysate; RNF111 transfected 293T cell lysate; Human prostate tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab88535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 109 kDa.
IHC-P Use a concentration of 3 µg/ml.

Target

  • FunctionActs in the NODAL pathway of mesoderm patterning during embryonic development. Acts downstream AXIN1 as an E3 ubiquitin-protein ligase which promotes the ubiquitination of inhibitory SMADs such as SMAD7, induces their proteasomal degradation and thereby enhances the transcriptional activity of TGF-beta and BMP. Activates Smad3/Smad4-dependent transcription by triggering signal-induced SnoN degradation.
  • Tissue specificityBroadly expressed.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 RING-type zinc finger.
  • Cellular localizationNucleus. Cytoplasm. Upon TGF-beta treatment, translocates from nucleus to cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARK antibody
    • Arkadia antibody
    • DKFZp313E0731 antibody
    • DKFZp686H1966 antibody
    • DKFZp761D081 antibody
    • E3 ubiquitin protein ligase Arkadia antibody
    • E3 ubiquitin-protein ligase Arkadia antibody
    • FLJ38008 antibody
    • RING finger protein 111 antibody
    • RN111_HUMAN antibody
    • RNF 111 antibody
    • RNF111 antibody
    see all

Anti-RNF111 antibody images

  • Anti-RNF111 antibody (ab88535) at 1 µg/ml + Rat brain lysate at 50 µg

    Predicted band size : 109 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-RNF111 antibody (ab88535) at 1 µg/ml

    Lane 1 : RNF111 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 109 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 125 kDa,230 kDa. We are unsure as to the identity of these extra bands.
  • ab88535, at 3µg/ml, staining RNF111 in formalin-fixed paraffin-embedded Human prostate tissue.

References for Anti-RNF111 antibody (ab88535)

ab88535 has not yet been referenced specifically in any publications.

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