Overview

  • Product nameAnti-RNF12 antibody
    See all RNF12 primary antibodies
  • Description
    Rabbit polyclonal to RNF12
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1 - 50 (MENSDSNDKG SGDQSAAQRR SQMDRLDREE AFYQFVNNLS EEDYRLMRDN) of Human RNF12 (NP_057204).

  • Positive control
    • HT1080 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab97838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionE3 ubiquitin-protein ligase. Acts as a negative coregulator for LIM homeodomain transcription factors by mediating the ubiquitination and subsequent degradation of LIM cofactors LDB1 and LDB2 and by mediating the recruitment the SIN3a/histone deacetylase corepressor complex. Ubiquitination and degradation of LIM cofactors LDB1 and LDB2 allows DNA-bound LIM homeodomain transcription factors to interact with other protein partners such as RLIM. Plays a role in telomere length-mediated growth suppression by mediating the ubiquitination and degradation of TERF1. By targeting ZFP42 for degradation, acts as an activator of random inactivation of X chromosome in the embryo, a stochastic process in which one X chromosome is inactivated to minimize sex-related dosage differences of X-encoded genes in somatic cells of female placental mammals.
  • Tissue specificityExpressed in many tissues.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the RNF12 family.
    Contains 1 RING-type zinc finger.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase RLIM antibody
    • LIM domain-interacting RING finger protein antibody
    • R-LIM antibody
    • Renal carcinoma antigen NY-REN-43 antibody
    • RING finger LIM domain-binding protein antibody
    • RING finger protein 12 antibody
    • RLIM antibody
    • RNF12 antibody
    • RNF12_HUMAN antibody
    see all

Anti-RNF12 antibody images

  • Anti-RNF12 antibody (ab97838) at 1 µg/ml (in 5% skim milk / PBS buffer) + HT1080 cell lysate

    Secondary
    HRP-conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 68 kDa

References for Anti-RNF12 antibody (ab97838)

ab97838 has not yet been referenced specifically in any publications.

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