Overview

  • Product name
    Anti-RNF121 antibody
  • Description
    Rabbit polyclonal to RNF121
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide: LFSAVTAFVT FRATRKPLVQ TTPRLVYKWF LLIYKISYAT GIVGYMAVMF, corresponding to amino acids 110-159 of Human RNF121

  • Positive control
    • Jurkat cell lysate and human intestine tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

Target

  • Relevance
    RNF121 protein contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. Three of them are supported by at least two independent transcripts or ESTs, the full length natures of others are not clear.
  • Cellular localization
    Membrane; Multi-pass membrane protein
  • Database links
  • Alternative names
    • 4930544L10Rik antibody
    • FLJ11099 antibody
    • ring finger protein 121 antibody

Anti-RNF121 antibody images

  • Anti-RNF121 antibody (ab59152) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling RNF121 with ab59152 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the intestinal villus. Magnifiaction: 400X.

References for Anti-RNF121 antibody (ab59152)

ab59152 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab59152.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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