Overview

  • Product nameAnti-RNF123 antibody
    See all RNF123 primary antibodies
  • Description
    Mouse monoclonal to RNF123
  • Tested applicationsSuitable for: WB, IP, IHC-FoFr, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment: ADYISADELA QVEQMLAHLT SASAQAAAAS LPTSEEDLCP ICYAHPISAV FQPCGHKSCK ACINQHLMNN KDCFFCKTTI VSVEDWEKGA NTSTTSSAA, corresponding to amino acids 1216-1315 of Human RNF123

Properties

Applications

Our Abpromise guarantee covers the use of ab57549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 149 kDa.
IP Use at an assay dependent concentration. PubMed: 21229311
IHC-FoFr Use at an assay dependent concentration. PubMed: 21229311
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionCatalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications
    Ubiquitinated, leading to its degradation. Deubiquitinated by USP19, thereby stimulating CDKN1B ubiquitin-dependent degradation.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686C2222 antibody
    • E3 ubiquitin protein ligase RNF123 antibody
    • E3 ubiquitin-protein ligase RNF123 antibody
    • FLJ12565 antibody
    • FP1477 antibody
    • Kip1 ubiquitination promoting complex protein 1 antibody
    • Kip1 ubiquitination-promoting complex protein 1 antibody
    • KPC 1 antibody
    • KPC1 antibody
    • MGC163504 antibody
    • RING finger protein 123 antibody
    • RN123_HUMAN antibody
    • RNF 123 antibody
    • Rnf123 antibody
    • Ubiquitin ligase antibody
    see all

Anti-RNF123 antibody images



  • Predicted band size : 149 kDa
    RNF123 antibody (ab57549) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab57549 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57549, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-RNF123 antibody (ab57549)

This product has been referenced in:
  • Rodrigo-Brenni MC  et al. Cytosolic quality control of mislocalized proteins requires RNF126 recruitment to Bag6. Mol Cell 55:227-37 (2014). Human . Read more (PubMed: 24981174) »
  • Zhao J  et al. KPC1 expression and essential role after acute spinal cord injury in adult rat. Neurochem Res 36:549-58 (2011). WB, IHC-FoFr, IP ; Rat . Read more (PubMed: 21229311) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Embryonic Fibroblast)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 10 µg
Specification Embryonic Fibroblast
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 06 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"