Anti-RNF125 antibody (ab74373)

Overview

  • Product nameAnti-RNF125 antibody
    See all RNF125 primary antibodies
  • Description
    Rabbit polyclonal to RNF125
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from an internal region of human RNF125.

  • Positive control
    • Extracts from K562 and HT-29 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab74373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

Target

  • RelevanceRNF125 is a novel E3 ubiquitin ligase that is expressed in CD4 and CD8 positive T cells and contains an N-terminal RING finger domain. It may function as a positive regulator in the T-cell receptor signaling pathway. E3 ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins.
  • Database links
  • Alternative names
    • E3 ubiquitin protein ligase RNF125 antibody
    • Ring finger protein 125 antibody
    • RNF 125 antibody
    • T cell RING activation protein 1 antibody
    • T cell ring protein identified in activation screen antibody
    • TRAC 1 antibody
    see all

Anti-RNF125 antibody images

  • All lanes : Anti-RNF125 antibody (ab74373) at 1/200 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : Mouse ES (129Sv) whole cell lysate

    Lysates/proteins at 5 µg per lane.

    Secondary
    HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 26 kDa
    Additional bands at : 100 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • All lanes : Anti-RNF125 antibody (ab74373) at 1/500 dilution

    Lane 1 : Extracts from K562 cells
    Lane 2 : Extracts from HT-29 cells
    Lane 3 : Extracts from K562 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 26 kDa
    Observed band size : 26 kDa
    Additional bands at : 100 kDa,23 kDa. We are unsure as to the identity of these extra bands.

References for Anti-RNF125 antibody (ab74373)

ab74373 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Mouse Cell lysate - whole cell (mouse ES cells)
Specification mouse ES cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Verified customer

Submitted Nov 11 2013

Thank you for taking the time to contact us. Your enquiries havebeen forwarded to the scientificsupport team.I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"