Overview

  • Product nameAnti-RNF128 antibody
    See all RNF128 primary antibodies
  • Description
    Rabbit polyclonal to RNF128
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: HVQSTNESLQ LVNHEANSVA VDVIPHVDNP TFEEDETPNQ ETAVREIKS, corresponding to C terminal amino acids 354-402 of Human RNF128

  • Positive control
    • HepG2 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab59150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay:1/312500.

Target

  • FunctionE3 ubiquitin-protein ligase that catalyzes polyubiquitin chains. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription and this activity is likely to be mediated by E3 ligase activity. Plays an important role in the induction of the anergic phenotype. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 PA (protease associated) domain.
    Contains 1 RING-type zinc finger.
  • DomainBinding to E2 ubiquitin-conjugating enzyme requires an intact RING finger domain.
  • Post-translational
    modifications
    Auto-ubiquitinated. Controls the development of T cell clonal anergy by ubiquitination.
  • Cellular localizationEndomembrane system. Cytoplasm > perinuclear region. Localized in an asymmetric perinuclear punctate manner. Localizes to the internal pool of the transferrin recycling endosomal pathway. Partially colocalized with the endoplasmic reticulum resident HSPA5, with Golgi resident STX5, and with the late endosomal GTPase RAB7.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin protein ligase RNF128 antibody
    • E3 ubiquitin-protein ligase RNF128 antibody
    • FLJ23516 antibody
    • Gene related to anergy in lymphocytes protein antibody
    • GRAIL antibody
    • RING finger protein 128 antibody
    • RN128_HUMAN antibody
    • RNF128 antibody
    see all

Anti-RNF128 antibody images



  • Predicted band size : 47 kDa
    Western blot analysis of CD4 T cell lysates from WT mice (lane 1) WT mice treated with anti-CD3/CD28 (lane 2) WT mice treated with FK506 (lane 3) WT mice treated with anti-CD3/CD28 and FK506 (lane 4) TRPV1 KO mice (lane 5) TRPV1 KO mice treated with anti-CD3/CD28 (lane 6) TRPV1 KO mice treated with FK506 (lane 7) and TRPV1 KO mice treated with anti-CD3/CD28 and FK506 (lane 8) labeling RNF128 with ab59150 at 1:1000. A HRP-conjugated anti-rabbit (1:10000) was used as the secondary antibody.
  • Anti-RNF128 antibody (ab59150) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 47 kDa
    Observed band size : 47 kDa

References for Anti-RNF128 antibody (ab59150)

ab59150 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. Unfortunately, the protein sequence was not checked and we have no supporting data other than the actual WB results. Please rest assured that all of our products are guaranteed (Abpromise) to work in species and appli...

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Thank you for your enquiry. Please rest assured that the molecular weight band that we obtained was checked using the SWISSPROT database, as shown below. Therefore, we are sure that the band we obtained was the RNF128 protein band. http://www.ex...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"