Overview

  • Product name
  • Description
    Rabbit polyclonal to RNF13
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (ILAYNFENAS QTFDDLPARF GYRLPAEGLK GFLINSKPEN ACEPIVPPPV) of Human RNF13 (NP_009213).

  • Positive control
    • HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab85421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    E3 ubiquitin-protein ligase that may play a role in controlling cell proliferation.
  • Tissue specificity
    Widely expressed (at protein level). In normal pancreas, expressed in islets, but not in ducts, nor in acini (at protein level).
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 PA (protease associated) domain.
    Contains 1 RING-type zinc finger.
  • Domain
    The RING-type zinc finger domain is required for E3 ligase activity.
  • Post-translational
    modifications
    Auto-ubiquitinated.
  • Cellular localization
    Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane. Lysosome membrane. Nucleus inner membrane. Under certain conditions, relocalizes to recycling endosomes and to the inner nuclear membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase RNF13 antibody
    • FLJ93817 antibody
    • MGC13689 antibody
    • RING finger protein 13 antibody
    • Ring zinc finger protein antibody
    • RNF 13 antibody
    • Rnf13 antibody
    • RNF13_HUMAN antibody
    • RZF antibody
    see all

Images

  • Anti-RNF13 antibody (ab85421) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 43 kDa
    Observed band size : 43 kDa

References

ab85421 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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