Overview

  • Product nameAnti-RNF130 antibody
    See all RNF130 primary antibodies
  • Description
    Rabbit polyclonal to RNF130
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    A region within synthetic peptide: ALINVTVQEP GRGAPLTFRI DRGRYGLDSP KAEVRGQVLA PLPLHGVADH, corresponding to N terminal amino acids 37-86 of Human RNF130

  • Positive control
    • Fetal thymus lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab49218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:62500.

WB Use a concentration of 2.5 mg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceRNF130 contains a RING finger motif and is similar to g1, a Drosophila zinc-finger protein that is expressed in mesoderm and involved in embryonic development. The expression of the mouse counterpart was found to be upregulated in myeloblastic cells following IL3 deprivation, suggesting that this gene may regulate growth factor withdrawal-induced apoptosis of myeloid precursor cells.
  • Cellular localizationMembrane; single-pass type I membrane protein
  • Database links
  • Alternative names
    • 2510042A13Rik antibody
    • G1 related zinc finger protein antibody
    • G1rp antibody
    • G1RZFP antibody
    • GOLIATH antibody
    • GP antibody
    • MGC117241 antibody
    • MGC138647 antibody
    • MGC99542 antibody
    • R goliath antibody
    • RING finger protein 130 antibody
    see all

Anti-RNF130 antibody images

  • Anti-RNF130 antibody (ab49218) at 2.5 µg/ml + Fetal thymus lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)

References for Anti-RNF130 antibody (ab49218)

ab49218 has not yet been referenced specifically in any publications.

Product Wall

The peptide that was used for the immunogen was derived from the range of amino acids 250 - 300 of the human protein. The numbering of the amino acids refers to the sequence at the UniProt database entry for human RNF130, accession Q86XS8:

h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"