Overview

  • Product nameAnti-RNF138 antibody
    See all RNF138 primary antibodies
  • Description
    Rabbit polyclonal to RNF138
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to an internal sequence of Human RNF138.

  • Positive control
    • MCF7 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab92730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: 1/40000.
    WB: 1/500 - 1/1000. Predicted molecular weight: 28 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionE3 ubiquitin-protein ligase. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesContains 1 RING-type zinc finger.
    • DomainThe RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
    • Post-translational
      modifications
      Auto-ubiquitinated.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • Database links
    • Alternative names
      • E3 ubiquitin protein ligase RNF138 antibody
      • E3 ubiquitin-protein ligase RNF138 antibody
      • hNARF antibody
      • HSD 4 antibody
      • HSD4 antibody
      • MGC8758 antibody
      • NARF antibody
      • Nemo like kinase associated RING finger protein antibody
      • Nemo-like kinase-associated RING finger protein antibody
      • NLK associated RING finger protein antibody
      • NLK-associated RING finger protein antibody
      • RING finger protein 138 antibody
      • ring finger protein 138, E3 ubiquitin protein ligase antibody
      • RN138_HUMAN antibody
      • Rnf138 antibody
      • STRIN antibody
      see all

    Anti-RNF138 antibody images

    • All lanes : Anti-RNF138 antibody (ab92730) at 1/500 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : MCF7 cell lysate with immunizing peptide at 5 µg

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 28 kDa

    References for Anti-RNF138 antibody (ab92730)

    ab92730 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (Bis-Tris 4-12%)
    Sample Mouse Tissue lysate - whole (Multiple Tissues)
    Specification Multiple Tissues
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 28 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"