Overview

  • Product nameAnti-RNF139 antibody
    See all RNF139 primary antibodies
  • Description
    Mouse monoclonal to RNF139
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: HYFHALCLRK WLYIQDTCPM CHQKVYIEDD IKDNSNVSNN NGFIPPNETP EEAVREAAAE SDRELNEDDS TDCDDDVQRE RNGVIQHTGA AAEEFNDDTD , corresponding to amino acids 565-665 of Human RNF139

Properties

Applications

Our Abpromise guarantee covers the use of ab56924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceRNF139 is a multi-membrane spanning protein E3 ubiquitin ligase which is mutated in clear-cell renal carcinomas. It contains a sterol-sensing domain and a RING-H2 motif essential for ubiquitin ligase activity. It is a potential tumor suppressor for renal cell carcinoma. Human kidney cells are growth inhibited by RNF139. Inhibition is manifested by G2/M arrest and is dependent upon the ubiquitin ligase activity of the RING domain. Expression of RNF139 represses genes involved in cholesterol and fatty acid biosynthesis that are transcriptionally regulated by the sterol response element binding proteins (SREBPs). RNF139 modulation of SREBP activity comprises a novel regulatory link between growth control and the cholesterol/lipid homeostasis pathway.
    • Cellular localizationEndoplasmic reticulum membrane; Multi-pass membrane protein.
    • Database links
    • Alternative names
      • E3 ubiquitin-protein ligase RNF139 antibody
      • HRCA1 antibody
      • RCA1 antibody
      • Ring finger protein 139 antibody
      • Translocation in renal carcinoma on chromosome 8 protein antibody
      • TRC8 antibody
      see all

    Anti-RNF139 antibody images

    • RNF139 antibody (ab56924) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.


    • Predicted band size : 76 kDa
      RNF139 antibody (ab56924) at 1ug/lane + HepG2 cell lysate at 25ug/lane.

    References for Anti-RNF139 antibody (ab56924)

    This product has been referenced in:
    • Gimelli S  et al. The tumor suppressor gene TRC8/RNF139 is disrupted by a constitutional balanced translocation t(8;22)(q24.13;q11.21) in a young girl with dysgerminoma. Mol Cancer 8:52 (2009). IHC-P ; Human . Read more (PubMed: 19642973) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"