Overview

  • Product nameAnti-RNF139 antibody
    See all RNF139 primary antibodies
  • Description
    Rabbit polyclonal to RNF139
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 71-120 (SQRSLFKFYT YSSAFLLAAT SVLVNYYASL HIDFYGAYNT SAFGIELLPR) of Human RNF139 (NP_009149).

  • Positive control
    • MCF7 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab82647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/312500.

Target

  • RelevanceRNF139 is a multi-membrane spanning protein E3 ubiquitin ligase which is mutated in clear-cell renal carcinomas. It contains a sterol-sensing domain and a RING-H2 motif essential for ubiquitin ligase activity. It is a potential tumor suppressor for renal cell carcinoma. Human kidney cells are growth inhibited by RNF139. Inhibition is manifested by G2/M arrest and is dependent upon the ubiquitin ligase activity of the RING domain. Expression of RNF139 represses genes involved in cholesterol and fatty acid biosynthesis that are transcriptionally regulated by the sterol response element binding proteins (SREBPs). RNF139 modulation of SREBP activity comprises a novel regulatory link between growth control and the cholesterol/lipid homeostasis pathway.
  • Cellular localizationEndoplasmic reticulum membrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase RNF139 antibody
    • HRCA1 antibody
    • RCA1 antibody
    • Ring finger protein 139 antibody
    • Translocation in renal carcinoma on chromosome 8 protein antibody
    • TRC8 antibody
    see all

Anti-RNF139 antibody images

  • Anti-RNF139 antibody (ab82647) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 76 kDa
    Observed band size : 76 kDa

References for Anti-RNF139 antibody (ab82647)

ab82647 has not yet been referenced specifically in any publications.

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