Anti-RNF139 antibody - C-terminal (ab189173)

Overview

  • Product name
    Anti-RNF139 antibody - C-terminal
    See all RNF139 primary antibodies
  • Description
    Goat polyclonal to RNF139 - C-terminal
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human RNF139 aa 651-663 (C terminal) (Cysteine residue).
    Sequence:

    HTGAAAEEFN DDT


    Database link: Q8WU17

  • Positive control
    • Human placenta tissue; Human skeletal muscle lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab189173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 76 kDa.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Relevance
    RNF139 is a multi-membrane spanning protein E3 ubiquitin ligase which is mutated in clear-cell renal carcinomas. It contains a sterol-sensing domain and a RING-H2 motif essential for ubiquitin ligase activity. It is a potential tumor suppressor for renal cell carcinoma. Human kidney cells are growth inhibited by RNF139. Inhibition is manifested by G2/M arrest and is dependent upon the ubiquitin ligase activity of the RING domain. Expression of RNF139 represses genes involved in cholesterol and fatty acid biosynthesis that are transcriptionally regulated by the sterol response element binding proteins (SREBPs). RNF139 modulation of SREBP activity comprises a novel regulatory link between growth control and the cholesterol/lipid homeostasis pathway.
  • Cellular localization
    Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase RNF139 antibody
    • HRCA1 antibody
    • RCA1 antibody
    • Ring finger protein 139 antibody
    • Translocation in renal carcinoma on chromosome 8 protein antibody
    • TRC8 antibody
    see all

Images

  • Anti-RNF139 antibody - C-terminal (ab189173) at 0.5 µg/ml + Human skeletal muscle cell lysate in RIPA buffer at 35 µg/ml

    Developed using the ECL technique.

    Predicted band size: 76 kDa


    Exposure time: 1 hour
  • Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Human placenta tissue labeling RNF139 with ab189173 at 3.75µg/ml.

References

ab189173 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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