Overview

  • Product name
    Anti-RNF141 antibody
  • Description
    Rabbit polyclonal to RNF141
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 96-145 (RIMNLYQFIQ LYKDITSQAA GVLAQSSTSE EPDENSSSVT SCQASLWMGR) of Human RNF141 (NP_057506).

  • Positive control
    • HepG2 cell lysate; Human brain and kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab105520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 26 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Anti-RNF141 antibody (ab105520) at 2 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size : 26 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling RNF141 with ab105520 at 4-8µg/ml. Arrows indicate positively labelled neural cells. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling RNF141 with ab105520 at 4-8µg/ml. Arrows indicate positive labelled epithelial cells of the renal tubule. Magnification: 400X.

References

ab105520 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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