Overview

  • Product nameAnti-RNF144 antibody
    See all RNF144 primary antibodies
  • Description
    Rabbit polyclonal to RNF144
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human, Rhesus monkey
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal sequence of human RNF144.

  • Positive control
    • extracts from COLO cells This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa. IHC-P: Human prostate FFPE tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab75054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 33 kDa).
ELISA 1/40000.

Target

  • FunctionE3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the RBR family. RNF144 subfamily.
    Contains 1 IBR-type zinc finger.
    Contains 2 RING-type zinc fingers.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0161 antibody
    • Probable E3 ubiquitin protein ligase RNF144A antibody
    • Probable E3 ubiquitin-protein ligase RNF144A antibody
    • R144A_HUMAN antibody
    • Ring finger protein 144 antibody
    • RING finger protein 144A antibody
    • RNF 144 antibody
    • RNF 144A antibody
    • rnf144a antibody
    • UBCE7IP4 antibody
    • UbcM4 interacting protein 4 antibody
    • UbcM4-interacting protein 4 antibody
    • Ubiquitin conjugating enzyme 7 interacting protein 4 antibody
    • Ubiquitin-conjugating enzyme 7-interacting protein 4 antibody
    see all

Anti-RNF144 antibody images

  • IHC image of RNF144 staining in human prostate formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75054, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab75054 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab75054 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-RNF144 antibody (ab75054) at 1/500 dilution

    Lane 1 : extracts from COLO cells
    Lane 2 : extracts from COLO cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 33 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 86 kDa. We are unsure as to the identity of these extra bands.

References for Anti-RNF144 antibody (ab75054)

This product has been referenced in:
  • Ho SR  et al. RNF144A, an E3 ubiquitin ligase for DNA-PKcs, promotes apoptosis during DNA damage. Proc Natl Acad Sci U S A 111:E2646-55 (2014). Read more (PubMed: 24979766) »
  • Marzook H  et al. Metastasis-associated protein 1 drives tumor cell migration and invasion through transcriptional repression of RING finger protein 144A. J Biol Chem 287:5615-26 (2012). WB ; Human . Read more (PubMed: 22184113) »

See all 2 Publications for this product

Product Wall

Thanks for your reply with the images.

I was able to view the longer exposure image but the not the shorter exposure, I think the file was corrupted. However, I still got a good idea of the results with the long exposure. Here are my suggest...

Read More

Thank you for speaking with me today.

I have issued a free of charge replacement for ab75054 which is scheduled for dispatch today and should be with you tomorrow. Please aliquot the product into single use aliquots to prevent freeze thawing...

Read More

Thank you for your reply.

I would be happy to speak with you further about these antibodies. Just let me know when would be a convenient time for you.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"