Overview

  • Product name
  • Description
    Mouse polyclonal to RNF144
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length Human RNF144 (NP_055561.2)

  • Positive control
    • Rat brain tissue lysate, HeLa cell lysate, RNF144 transfected 293T cell lysate and HeLa cell

Properties

Applications

Our Abpromise guarantee covers the use of ab89260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 33 kDa.

Target

  • Function
    E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the RBR family. RNF144 subfamily.
    Contains 1 IBR-type zinc finger.
    Contains 2 RING-type zinc fingers.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0161 antibody
    • Probable E3 ubiquitin protein ligase RNF144A antibody
    • Probable E3 ubiquitin-protein ligase RNF144A antibody
    • R144A_HUMAN antibody
    • Ring finger protein 144 antibody
    • RING finger protein 144A antibody
    • RNF 144 antibody
    • RNF 144A antibody
    • rnf144a antibody
    • UBCE7IP4 antibody
    • UbcM4 interacting protein 4 antibody
    • UbcM4-interacting protein 4 antibody
    • Ubiquitin conjugating enzyme 7 interacting protein 4 antibody
    • Ubiquitin-conjugating enzyme 7-interacting protein 4 antibody
    see all

Images

  • Anti-RNF144 antibody (ab89260) at 5 µg/ml + Rat brain tissue lysate at 50 µg

    Predicted band size : 33 kDa
  • Anti-RNF144 antibody (ab89260) at 5 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 33 kDa
  • All lanes : Anti-RNF144 antibody (ab89260) at 5 µg/ml

    Lane 1 : RNF144 transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 50 µg per lane.


    Predicted band size : 33 kDa
  • ab89260 at 10µg/ml staining RNF144 on HeLa cell by immunofluorescence

References

This product has been referenced in:
  • Ho SR  et al. Regulation of RNF144A E3 Ubiquitin Ligase Activity by Self-association through Its Transmembrane Domain. J Biol Chem 290:23026-38 (2015). Read more (PubMed: 26216882) »
  • Ho SR  et al. RNF144A, an E3 ubiquitin ligase for DNA-PKcs, promotes apoptosis during DNA damage. Proc Natl Acad Sci U S A 111:E2646-55 (2014). Read more (PubMed: 24979766) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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