Overview

  • Product name
  • Description
    Rabbit polyclonal to RNF146
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 300-349 (AVVAQHSLTQ QRLLVSNANQ TVPDRSDRSG TDRSVAGGGT VSVSVRSRRP) of Human RNF146 (NP_112225).

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab106334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated proteins and mediates their ubiquitination and subsequent degradation. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of poly-ADP-ribosylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate poly-ADP-ribosylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent poly-ADP-ribosylated proteins via its WWE domain and mediates their ubiquitination.
  • Tissue specificity
    Ubiquitously expressed. Up-regulated in brains from patients with Alzheimer disease.
  • Pathway
    Protein modification; protein ubiquitination.
  • Involvement in disease
    Note=Defects in RNF146 are a cause of susceptibility to breast cancer.
  • Sequence similarities
    Contains 1 RING-type zinc finger.
    Contains 1 WWE domain.
  • Domain
    The WWE domain mediates non-covalent poly(ADP-ribose)-binding.
  • Post-translational
    modifications
    Ubiquitinated; autoubiquitinated. Autoubiquitination is enhanced upon poly(ADP-ribose)-binding.
  • Cellular localization
    Cytoplasm > cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dactylidin antibody
    • dJ351K20.1 antibody
    • DKFZP434O1427 antibody
    • E3 ubiquitin-protein ligase rnf146 antibody
    • RING finger protein 146 antibody
    • RN146_HUMAN antibody
    • RNF 146 antibody
    • Rnf146 antibody
    • RP3 351K20.1 antibody
    see all

Images

  • Anti-RNF146 antibody (ab106334) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size : 39 kDa

References

ab106334 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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