The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 39 kDa).
FunctionE3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated proteins and mediates their ubiquitination and subsequent degradation. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of poly-ADP-ribosylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate poly-ADP-ribosylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent poly-ADP-ribosylated proteins via its WWE domain and mediates their ubiquitination.
Tissue specificityUbiquitously expressed. Up-regulated in brains from patients with Alzheimer disease.
PathwayProtein modification; protein ubiquitination.
Involvement in diseaseNote=Defects in RNF146 are a cause of susceptibility to breast cancer.
Sequence similaritiesContains 1 RING-type zinc finger. Contains 1 WWE domain.
DomainThe WWE domain mediates non-covalent poly(ADP-ribose)-binding.
Post-translational modificationsUbiquitinated; autoubiquitinated. Autoubiquitination is enhanced upon poly(ADP-ribose)-binding.