Overview

  • Product nameAnti-RNF146 antibody
    See all RNF146 primary antibodies
  • Description
    Mouse polyclonal to RNF146
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human RNF146 protein (NP_112225.2).

  • Positive control
    • RNF146 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab69228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 39 kDa).

Target

  • FunctionE3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated proteins and mediates their ubiquitination and subsequent degradation. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of poly-ADP-ribosylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate poly-ADP-ribosylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent poly-ADP-ribosylated proteins via its WWE domain and mediates their ubiquitination.
  • Tissue specificityUbiquitously expressed. Up-regulated in brains from patients with Alzheimer disease.
  • PathwayProtein modification; protein ubiquitination.
  • Involvement in diseaseNote=Defects in RNF146 are a cause of susceptibility to breast cancer.
  • Sequence similaritiesContains 1 RING-type zinc finger.
    Contains 1 WWE domain.
  • DomainThe WWE domain mediates non-covalent poly(ADP-ribose)-binding.
  • Post-translational
    modifications
    Ubiquitinated; autoubiquitinated. Autoubiquitination is enhanced upon poly(ADP-ribose)-binding.
  • Cellular localizationCytoplasm > cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dactylidin antibody
    • dJ351K20.1 antibody
    • DKFZP434O1427 antibody
    • E3 ubiquitin-protein ligase rnf146 antibody
    • RING finger protein 146 antibody
    • RN146_HUMAN antibody
    • RNF 146 antibody
    • Rnf146 antibody
    • RP3 351K20.1 antibody
    see all

Anti-RNF146 antibody images

  • All lanes : Anti-RNF146 antibody (ab69228) at 1/500 dilution

    Lane 1 : RNF146 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 39 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References for Anti-RNF146 antibody (ab69228)

ab69228 has not yet been referenced specifically in any publications.

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