Overview

  • Product name
  • Description
    Rabbit polyclonal to RNF149
  • Specificity
    Detects endogenous levels of total RNF149 protein.
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal region of Human RNF149.

  • Positive control
    • Human brain tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.88% Sodium chloride, 49.1% PBS
    Note: PBS (without Mg2+ and Ca2+)
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab111193 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 PA (protease associated) domain.
    Contains 1 RING-type zinc finger.
  • Domain
    The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA polymerase-transactivated protein 2 antibody
    • DNAPTP2 antibody
    • E3 ubiquitin-protein ligase RNF149 antibody
    • RING finger protein 149 antibody
    • RN149_HUMAN antibody
    • Rnf149 antibody
    see all

Anti-RNF149 antibody images

  • ab111193 at 1/50 dilution staining RNF149 in paraffin-embedded Human Brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized peptide.

References for Anti-RNF149 antibody (ab111193)

ab111193 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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