Overview

  • Product name
  • Description
    Rabbit polyclonal to RNF160
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 47-96 (MGGKNKQRTK GNLRPSNSGR AAELLAKEQG TVPGFIGFGT SQSDLGYVPA) of Human RNF160 (NP_056380)

  • Positive control
    • HepG2 cell lysate IF/ICC: HeLa cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab104375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 201 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    RNF160 may function as an E3 ubiquitin-protein ligase. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
  • Database links
  • Alternative names
    • C21orf10 antibody
    • C21orf98 antibody
    • E3 ubiquitin protein ligase listerin antibody
    • HSPC087 antibody
    • Kiaa0714 antibody
    • Lister antibody
    • Listerin E3 ubiquitin protein ligase 1 antibody
    • Ring finger protein 160 antibody
    • Zinc finger protein 294 antibody
    • ZNF294 antibody
    see all

Images

  • ICC/IF image of ab104375 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104375, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-RNF160 antibody (ab104375) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size : 201 kDa
    Gel concentration 6-18%

References

ab104375 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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