Recombinant fragment Predicted to work with:
Mouse, Rat, Horse, Chicken, Cow, Dog, Human, Pig
Recombinant fragment: MASQPPEDTA ESQASDELEC KICYNRYNLK QRKPKVLECC HRVCAKCLYK IIDFGDSPQG VIVCPFCRFE TCLPDDEVSS LPDDNNILVN LTCGGKGKK, corresponding to amino acids 1-100 of human RNF182 (NP_689950) with a tag.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 27 kDa.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionE3 ubiquitin-protein ligase that mediates the ubiquitination of ATP6V0C and targets it to degradation via the ubiquitin-proteasome pathway.
Tissue specificityUp-regulated in neuronal cells subjected to cell death-inducing injuries, such as oxygen and glucose deprivation (at protein level). Could be up-regulated in Alzheimer disease brains.
PathwayProtein modification; protein ubiquitination.