Overview

  • Product nameAnti-RNF182 antibody
    See all RNF182 primary antibodies
  • Description
    Mouse monoclonal to RNF182
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Human, Pig
  • Immunogen

    Recombinant fragment: MASQPPEDTA ESQASDELEC KICYNRYNLK QRKPKVLECC HRVCAKCLYK IIDFGDSPQG VIVCPFCRFE TCLPDDEVSS LPDDNNILVN LTCGGKGKK, corresponding to amino acids 1-100 of human RNF182 (NP_689950) with a tag.

  • Positive control
    • Recombinant tagged human RNF182 fragment (the immunogen).

Properties

Applications

Our Abpromise guarantee covers the use of ab76796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 27 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-RNF182 antibody images

    • Anti-RNF182 antibody (ab76796) at 5 µg/ml + recombinant tagged human RNF182 fragment at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 27 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)

    References for Anti-RNF182 antibody (ab76796)

    ab76796 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76796.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"