Overview

  • Product name
  • Description
    Mouse polyclonal to RNF185
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human RNF185 protein (NP_689480)

  • Positive control
    • RNF185 transfected 293T cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Purified IgA
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab73118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 20 kDa.

Target

  • Relevance
    RNF185 contains 1 RING-type zinc finger. The exact function of RNF185 remains unknown. There are 2 named isoforms produced by alternative splicing.
  • Cellular localization
    Mitochondrion outer membrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • E3 ubiquitin protein ligase RNF185 antibody
    • FLJ38628 antibody
    • Ring finger protein 185 antibody

Images

  • All lanes : Anti-RNF185 antibody (ab73118) at 1/500 dilution

    Lane 1 : RNF185 transfected 293T cell lysate
    Lane 2 : non transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 20 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)

References

ab73118 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab73118.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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