Overview

  • Product name
    Anti-RNF187 antibody [5H10]
    See all RNF187 primary antibodies
  • Description
    Mouse monoclonal [5H10] to RNF187
  • Tested applications
    Suitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Single C-terminal immunogenic peptide

  • Epitope
    aa174-189.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    5H10
  • Myeloma
    Sp2
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 15 kDa.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • Function
    E3 ubiquitin-protein ligase that acts as a coactivator of JUN-mediated gene activation in response to growth factor signaling via the MAP3K1 pathway, independently from MAPK8.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 RING-type zinc finger.
  • Domain
    The RING-type zinc finger domain is required for E3 ligase activity.
  • Post-translational
    modifications
    Ubiquitinated; undergoes 'Lys-48'-linked autoubiquitination in the absence of growth factors and MAP3K1-induced 'Lys-63'-linked polyubiquitination. 'Lys-48'-autoubiquitination leads to degradation by the proteasome, while MAP3K1-induced 'Lys-63'-linked polyubiquitination results in the stabilization of the protein. 'Lys-48'- and 'Lys-63'-linked polyubiquitinations occur most probably on the same 3 C-terminal lysine residues (Lys-195, Lys-223 and Lys-224) and are thus mutually exclusive. Other sites of ubiquitination are not excluded.
    Arginine methylation by PRMT1 stabilizes RNF187 by facilitating K63-linked ubiquitin chain formation, and enables dimerization, c-Jun interaction and subsequent AP1 target gene expression.
  • Cellular localization
    Cytoplasm. Nucleus. Predominantly located in the cytoplasm. Shuttles between the cytoplasm and the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase RNF187 antibody
    • Protein RNF187 antibody
    • RACO-1 antibody
    • RACO1 antibody
    • RING domain AP1 coactivator 1 antibody
    • RING finger protein 187 antibody
    • RN187_HUMAN antibody
    • RNF187 antibody
    see all

References

This product has been referenced in:
  • Davies CC  et al. Identification of a co-activator that links growth factor signalling to c-Jun/AP-1 activation. Nat Cell Biol 12:963-72 (2010). Read more (PubMed: 20852630) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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