The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/200. Use with methanol fixed cells. This antibody does not appear to be compatible with paraformaldehyde fixation.
WB: 1/500. ab33500 can show some non-specific bands - anyone wanting to use this antibody for WB should consider image below or abreview.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
Expressed in the normal brain and also in malignant gliomas (at protein level).
Protein modification; protein ubiquitination.
Belongs to the BRE1 family. Contains 1 RING-type zinc finger.
Ring finger protein 20 E3 ubiquitin protein ligase antibody
Immunocytochemistry/ Immunofluorescence - RNF20 antibody (ab33500)This image is courtesy of an Abreview submitted by Dr Kirk McManus
ab33500 (1/500) detecting RNF20 in HeLa cells (green). Cells were fixed with methanol and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.