The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 114 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionComponent of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
Tissue specificityExpressed in the normal brain and also in malignant gliomas (at protein level).
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the BRE1 family. Contains 1 RING-type zinc finger.
Detection of Human RNF20 by Western Blot of Immunprecipitate. ab70496 at 1µg/ml staining RNF20 in HeLa whole cell lysates immunoprecipitated at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) using ab70496. Detection: Chemiluminescence with exposure times of 10 seconds.
References for Anti-RNF20 antibody (ab70496)
has not yet been referenced specifically in any publications.
Publishing research using ab70496? Please let us know so that we can cite the reference in this datasheet.
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