Overview

  • Product nameAnti-RNF20 antibody
    See all RNF20 primary antibodies
  • Description
    Rabbit polyclonal to RNF20
  • SpecificityWe have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 125 and 175 of human RNF20. (NP_062538.5)

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab70496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 114 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
    • Tissue specificityExpressed in the normal brain and also in malignant gliomas (at protein level).
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesBelongs to the BRE1 family.
      Contains 1 RING-type zinc finger.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • BRE1 A antibody
      • BRE1 antibody
      • BRE1 E3 ubiquitin ligase homolog antibody
      • BRE1-A antibody
      • BRE1A antibody
      • BRE1A_HUMAN antibody
      • E3 ubiquitin-protein ligase BRE1A antibody
      • hBRE1 antibody
      • Homolog of S. cerevisiae BRE1 antibody
      • RING finger protein 20 antibody
      • Ring finger protein 20 E3 ubiquitin protein ligase antibody
      • Rnf20 antibody
      see all

    Anti-RNF20 antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human metastatic lymph node tissue labelling RNF20 with ab70496 at 1/1000 (1µg/ml). Detection: DAB.
    • All lanes : Anti-RNF20 antibody (ab70496) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 114 kDa
      Observed band size : 125 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds
    • Detection of Human RNF20 by Western Blot of Immunprecipitate. ab70496 at 1µg/ml staining RNF20 in HeLa whole cell lysates immunoprecipitated at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) using ab70496. Detection: Chemiluminescence with exposure times of 10 seconds.

    References for Anti-RNF20 antibody (ab70496)

    ab70496 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"