Overview

  • Product nameAnti-RNF20 antibody
    See all RNF20 primary antibodies
  • Description
    Mouse polyclonal to RNF20
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human RNF20, amino acids 1-975 (NP_062538.5)

  • Positive control
    • HeLa and HL60 cell lysates, RNF20 transfected 293T cell lysate and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab89764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 114 kDa.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • FunctionComponent of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
  • Tissue specificityExpressed in the normal brain and also in malignant gliomas (at protein level).
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the BRE1 family.
    Contains 1 RING-type zinc finger.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRE1 A antibody
    • BRE1 antibody
    • BRE1 E3 ubiquitin ligase homolog antibody
    • BRE1-A antibody
    • BRE1A antibody
    • BRE1A_HUMAN antibody
    • E3 ubiquitin-protein ligase BRE1A antibody
    • hBRE1 antibody
    • Homolog of S. cerevisiae BRE1 antibody
    • RING finger protein 20 antibody
    • Ring finger protein 20 E3 ubiquitin protein ligase antibody
    • Rnf20 antibody
    see all

Anti-RNF20 antibody images

  • Anti-RNF20 antibody (ab89764) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 114 kDa
    Observed band size : 114 kDa
  • Anti-RNF20 antibody (ab89764) at 1 µg/ml + HL60 cell lysate at 50 µg

    Predicted band size : 114 kDa
    Observed band size : 114 kDa
  • All lanes : Anti-RNF20 antibody (ab89764) at 1 µg/ml

    Lane 1 : RNF20 transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 114 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
  • ab89764 at 20µg/ml staining RNF20 in HeLa cells by Immunofluorescence.

References for Anti-RNF20 antibody (ab89764)

ab89764 has not yet been referenced specifically in any publications.

Product Wall

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