Recombinant
RabMAb

Anti-RNF20 antibody [EPR13562(B)] (ab181104)

Overview

  • Product name
    Anti-RNF20 antibody [EPR13562(B)]
    See all RNF20 primary antibodies
  • Description
    Rabbit monoclonal [EPR13562(B)] to RNF20
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IP, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human RNF20 aa 1-100. The exact sequence is proprietary.
    Database link: Q5VTR2

  • Positive control
    • Jurkat, 293, MCF7 and HeLa cell lysates,
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR13562(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IP 1/50.
Flow Cyt 1/290.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/2000. Predicted molecular weight: 114 kDa.

Target

  • Function
    Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
  • Tissue specificity
    Expressed in the normal brain and also in malignant gliomas (at protein level).
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the BRE1 family.
    Contains 1 RING-type zinc finger.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRE1 A antibody
    • BRE1 antibody
    • BRE1 E3 ubiquitin ligase homolog antibody
    • BRE1-A antibody
    • BRE1A antibody
    • BRE1A_HUMAN antibody
    • E3 ubiquitin-protein ligase BRE1A antibody
    • hBRE1 antibody
    • Homolog of S. cerevisiae BRE1 antibody
    • RING finger protein 20 antibody
    • Ring finger protein 20 E3 ubiquitin protein ligase antibody
    • Rnf20 antibody
    see all

Images

  • Immonofluorescence analysis of 4% paraformaldehyde fixed MCF7 cells, labeling RNF20 using ab181104 at a 1/250 dilution (green) followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at a 1/2000 dilution and counterstained with Dapi (blue).

  • Flow cytometry analysis of 2% paraformaldehyde fixed Jurkat cells labeling RNF20 with ab181104 at 1/290 dilution (red) or a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) at a 1/150 dilution.

  • Western blot analysis on immunoprecipitation of 293 cell lysate labeling RNF20 using ab181104 at a 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution and 5% NFDM/TBST blocking peptide.

  • All lanes : Anti-RNF20 antibody [EPR13562(B)] (ab181104) at 1/5000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 114 kDa

References

ab181104 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181104.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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