• Product nameAnti-RNF20 antibody [RNF20-85]
    See all RNF20 primary antibodies
  • Description
    Mouse monoclonal [RNF20-85] to RNF20
  • Tested applicationsSuitable for: Indirect ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cynomolgus Monkey
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 540-565 of Human RNF20

  • Positive control
    • HeLa cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purification notesAb50534 is a purified immunoglobulin.
  • ClonalityMonoclonal
  • Clone numberRNF20-85
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab50534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 114 kDa.
ICC/IF Use a concentration of 10 - 20 µg/ml.


  • FunctionComponent of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
  • Tissue specificityExpressed in the normal brain and also in malignant gliomas (at protein level).
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the BRE1 family.
    Contains 1 RING-type zinc finger.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRE1 A antibody
    • BRE1 antibody
    • BRE1 E3 ubiquitin ligase homolog antibody
    • BRE1-A antibody
    • BRE1A antibody
    • BRE1A_HUMAN antibody
    • E3 ubiquitin-protein ligase BRE1A antibody
    • hBRE1 antibody
    • Homolog of S. cerevisiae BRE1 antibody
    • RING finger protein 20 antibody
    • Ring finger protein 20 E3 ubiquitin protein ligase antibody
    • Rnf20 antibody
    see all

Anti-RNF20 antibody [RNF20-85] images

  • HeLa cells, fixed with 4% paraformaldehyde for 10 minutes. Permeabilized with 0.5% triton X-100 0.5% for 5 minutes. Ab50534 used at a concentration of 10 microgram/ml with 1%BSA for 1 hour.

References for Anti-RNF20 antibody [RNF20-85] (ab50534)

This product has been referenced in:
  • Kelly E  et al. Selenoprotein S/SEPS1 modifies endoplasmic reticulum stress in Z variant alpha1-antitrypsin deficiency. J Biol Chem 284:16891-7 (2009). WB ; Human . Read more (PubMed: 19398551) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100

Dr. Kirk McManus

Verified customer

Submitted Oct 19 2010