Overview

  • Product name
  • Description
    Goat polyclonal to RNF22
  • Specificity
    ab115606 is expected to recognise all three human isoforms of this protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog
  • Immunogen

    Synthetic peptide:

    AKREDSPGPEVQP

    with a C terminal Cysteine residue linker, corresponding to N terminal amino acids 2-14 of Human RNF22 (NP_006449.2).

  • Positive control
    • Human Adrenal tissue; Mouse brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab115606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 81 kDa.
ELISA 1/64000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
  • Tissue specificity
    Expressed in brain, heart, uterus and testis.
  • Sequence similarities
    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 filamin repeat.
    Contains 6 NHL repeats.
    Contains 1 RING-type zinc finger.
  • Domain
    The interaction with MYO5B is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.
  • Cellular localization
    Cytoplasm. Early endosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • BERP antibody
    • Brain expressed ring finger antibody
    • Brain-expressed RING finger protein antibody
    • FLJ16135 antibody
    • HAC1 antibody
    • RING finger protein 22 antibody
    • RING finger protein 97 antibody
    • RNF22 antibody
    • RNF97 antibody
    • Trim3 antibody
    • TRIM3_HUMAN antibody
    • Tripartite motif containing 3 antibody
    • Tripartite motif protein TRIM3 antibody
    • Tripartite motif-containing protein 3 antibody
    see all

Images

  • Anti-RNF22 antibody (ab115606) at 1 µg/ml + Mouse brain lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 81 kDa
  • ab115606, at 5µg/ml, staining RNF22 in Formalin-fixed, Paraffin-embedded Human Adrenal tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

ab115606 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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