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Goat polyclonal to RNF22
ab115606 is expected to recognise all three human isoforms of this protein.
Predicted to work with:
Mouse, Rat, Horse, Cow, Dog
with a C terminal Cysteine residue linker, corresponding to N terminal amino acids 2-14 of Human RNF22 (NP_006449.2).
Human Adrenal tissue; Mouse brain lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
Protein G purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 81 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Expressed in brain, heart, uterus and testis.
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger. Contains 1 filamin repeat. Contains 6 NHL repeats. Contains 1 RING-type zinc finger.
The interaction with MYO5B is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.
Cytoplasm. Early endosome.
Information by UniProt
Brain expressed ring finger antibody
Brain-expressed RING finger protein antibody
Western blot - Anti-RNF22 antibody (ab115606)
Anti-RNF22 antibody (ab115606) at 1 µg/ml + Mouse brain lysate at 35 µg
Developed using the ECL technique. Predicted band size: 81 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF22 antibody (ab115606)
ab115606, at 5µg/ml, staining RNF22 in Formalin-fixed, Paraffin-embedded Human Adrenal tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
has not yet been referenced specifically in any publications.
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