The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 0.1 - 1 µg/ml. Approx 85kDa band seen in Mouse Brain and Human Brain (Cerebellum) lysate [Predicted MW of approx. 80.8kDa according to NP_006449.2].
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionProbably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Tissue specificityExpressed in brain, heart, uterus and testis.
Sequence similaritiesBelongs to the TRIM/RBCC family. Contains 1 B box-type zinc finger. Contains 1 filamin repeat. Contains 6 NHL repeats. Contains 1 RING-type zinc finger.
DomainThe interaction with MYO5B is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.
Immunoprecipitation - RNF22 antibody (ab4215)This image is courtesy of an Abreview submitted by Dr Christoph Maas
ab4215 immunoprecipitating RNF22 from rat brain tissue whole lysate. The antibody was used at a concentration of 2.5ug/mg of lysate. A Protein G matrix was used. The same antibody was used in the Western blot stage of the experiment (left hand panel).