Overview

  • Product name
  • Description
    Goat polyclonal to RNF22
  • Specificity
    This antibody is expected to recognise both human isoforms.
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide:

    AKREDSPGPEVQP-C

    , corresponding to N terminal amino acids 2-14 of Human RNF22 (Peptide available as ab23010.)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
  • Application notes
    ELISA: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.1 - 1 µg/ml. Approx 85kDa band seen in Mouse Brain and Human Brain (Cerebellum) lysate [Predicted MW of approx. 80.8kDa according to NP_006449.2].


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
    • Tissue specificity
      Expressed in brain, heart, uterus and testis.
    • Sequence similarities
      Belongs to the TRIM/RBCC family.
      Contains 1 B box-type zinc finger.
      Contains 1 filamin repeat.
      Contains 6 NHL repeats.
      Contains 1 RING-type zinc finger.
    • Domain
      The interaction with MYO5B is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.
    • Cellular localization
      Cytoplasm. Early endosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • BERP antibody
      • Brain expressed ring finger antibody
      • Brain-expressed RING finger protein antibody
      • FLJ16135 antibody
      • HAC1 antibody
      • RING finger protein 22 antibody
      • RING finger protein 97 antibody
      • RNF22 antibody
      • RNF97 antibody
      • Trim3 antibody
      • TRIM3_HUMAN antibody
      • Tripartite motif containing 3 antibody
      • Tripartite motif protein TRIM3 antibody
      • Tripartite motif-containing protein 3 antibody
      see all

    Anti-RNF22 antibody images

    • All lanes : Anti-RNF22 antibody (ab4215) at 0.3 µg/ml

      Lane 1 : Human cerebellum lysate (RIPA Buffer)
      Lane 2 : Rat brain lysate (RIPA Buffer)

      Lysates/proteins at 35 µg per lane.


      Predicted band size : 81 kDa
    • Anti-RNF22 antibody (ab4215) at 0.5 µg/ml + Mouse Brain (RIPA buffer) at 30 µg

      Predicted band size : 81 kDa
      Observed band size : 85 kDa (why is the actual band size different from the predicted?)
      Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
    • ab4215 immunoprecipitating RNF22 from rat brain tissue whole lysate. The antibody was used at a concentration of 2.5ug/mg of lysate. A Protein G matrix was used. The same antibody was used in the Western blot stage of the experiment (left hand panel).

      See Abreview

    References for Anti-RNF22 antibody (ab4215)

    ab4215 has not yet been referenced specifically in any publications.

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    Filter by Ratings

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Rat Tissue lysate - whole (Brain)
    Total protein in input
    500 µg
    Specification
    Brain
    Immuno-precipitation step
    Protein G
    Username

    忠義 大塚

    Verified customer

    Submitted Jun 27 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Brain)
    Loading amount
    30 µg
    Specification
    Brain
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    忠義 大塚

    Verified customer

    Submitted Jun 27 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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