Overview

  • Product name
  • Description
    Rabbit polyclonal to RNF22
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 50 and 100 of human RNF22 (NP_006449.2)

  • Positive control
    • HeLa whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab74190 was affinity purified using an epitope specific to RNF22 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
    • Tissue specificity
      Expressed in brain, heart, uterus and testis.
    • Sequence similarities
      Belongs to the TRIM/RBCC family.
      Contains 1 B box-type zinc finger.
      Contains 1 filamin repeat.
      Contains 6 NHL repeats.
      Contains 1 RING-type zinc finger.
    • Domain
      The interaction with MYO5B is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.
    • Cellular localization
      Cytoplasm. Early endosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • BERP antibody
      • Brain expressed ring finger antibody
      • Brain-expressed RING finger protein antibody
      • FLJ16135 antibody
      • HAC1 antibody
      • RING finger protein 22 antibody
      • RING finger protein 97 antibody
      • RNF22 antibody
      • RNF97 antibody
      • Trim3 antibody
      • TRIM3_HUMAN antibody
      • Tripartite motif containing 3 antibody
      • Tripartite motif protein TRIM3 antibody
      • Tripartite motif-containing protein 3 antibody
      see all

    Images

    • Detection of RNF22 by Western Blot of Immunprecipitate.
      anit-RNF22 (ab74191) at 1µg/ml staining RNF22 in HeLa whole cell lysate immunoprecipitated using ab74190 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 30 seconds.

    References

    ab74190 has not yet been referenced specifically in any publications.

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    There are currently no Customer reviews or Questions for ab74190.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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