Overview

  • Product nameAnti-RNF22 antibody
    See all RNF22 primary antibodies
  • Description
    Rabbit polyclonal to RNF22
  • Tested applicationsSuitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Synthetic peptide corresponding to a region between residues 400 and 450 of human RNF22 (NP_006449.2)

  • Positive control
    • HeLa whole cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab74191 was affinity purified using an epitope specific to RNF22 immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab74191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionProbably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
    • Tissue specificityExpressed in brain, heart, uterus and testis.
    • Sequence similaritiesBelongs to the TRIM/RBCC family.
      Contains 1 B box-type zinc finger.
      Contains 1 filamin repeat.
      Contains 6 NHL repeats.
      Contains 1 RING-type zinc finger.
    • DomainThe interaction with MYO5B is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.
    • Cellular localizationCytoplasm. Early endosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • BERP antibody
      • Brain expressed ring finger antibody
      • Brain-expressed RING finger protein antibody
      • FLJ16135 antibody
      • HAC1 antibody
      • RING finger protein 22 antibody
      • RING finger protein 97 antibody
      • RNF22 antibody
      • RNF97 antibody
      • Trim3 antibody
      • TRIM3_HUMAN antibody
      • Tripartite motif containing 3 antibody
      • Tripartite motif protein TRIM3 antibody
      • Tripartite motif-containing protein 3 antibody
      see all

    Anti-RNF22 antibody images

    • Detection of RNF22 by Western Blot of Immunprecipitate.
      ab74191 at 1µg/ml staining RNF22 in HeLa whole cell lysate immunoprecipitated using ab74191 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 30 seconds.

    References for Anti-RNF22 antibody (ab74191)

    ab74191 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Rat Tissue lysate - other (cortex lysate differentially centrifuged, P3)
    Loading amount 5 µg
    Specification cortex lysate differentially centrifuged, P3
    Gel Running Conditions Reduced Denaturing (8%)
    Blocking step Serum as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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    Submitted Sep 28 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"