Overview

  • Product name
    Anti-RNF220 antibody
  • Description
    Mouse polyclonal to RNF220
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human RNF220 protein (AAH98300.1)

  • Positive control
    • RNF220 transfected 293T cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 63 kDa).

Target

  • Relevance
    RNF220 contains one RING-type zinc finger. There are two named isoforms.
  • Database links
  • Alternative names
    • C1orf164 antibody
    • Chromosome 1 open reading frame 164 antibody
    • FLJ10597 antibody
    • Ring finger protein 220 antibody
    • RING finger protein C1orf164 antibody
    • RNF 220 antibody
    • RP4 678E16.1 antibody
    see all

Images

  • All lanes : Anti-RNF220 antibody (ab69357) at 1/500 dilution

    Lane 1 : RNF220 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 63 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • ab69357 at 10µg/ml staining RNF220 in Hela cells by ICC/IF.

References

ab69357 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab69357.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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