Overview

  • Product nameAnti-RNF23 antibody
    See all RNF23 primary antibodies
  • Description
    Rabbit polyclonal to RNF23
  • SpecificityThis antibody is specific for RNF23.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Chimpanzee
  • Immunogen

    A region within synthetic peptide: QHHEALSLFC YEDQEAVCLI CAISHTHRAH TVVPLDDATQ EYKEKLQKCL, corresponding to N terminal amino acids 109-158 of Human RNF23

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab49312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 mg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:12500

Target

  • FunctionMay facilitate apoptosis by inhibiting the poly-ubiquitination and subsequent proteasome-mediated degradation of the pro-apoptotic protein MOAP1.
  • Tissue specificityUbiquitous; highly expressed in brain, heart, kidney, liver, skeletal muscle, spleen and testis.
  • Sequence similaritiesBelongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 RING-type zinc finger.
  • Cellular localizationCytoplasm > cytosol. Mitochondrion. Found predominantly in the cytosol. Partial shift from the cytosol to the mitochondria when co-localized with MOAP1.
  • Information by UniProt
  • Database links
  • Alternative names
    • HGNC:10065 antibody
    • MGC32984 antibody
    • RING finger protein 23 antibody
    • RNF23 antibody
    • Testis abundant finger protein antibody
    • Testis-abundant finger protein antibody
    • TFP antibody
    • TFP RING FINGER PROTEIN 23 antibody
    • TRI39_HUMAN antibody
    • Trim39 antibody
    • TRIM39B antibody
    • Tripartite motif containing 39 antibody
    • Tripartite motif-containing protein 39 antibody
    see all

Anti-RNF23 antibody images

  • Anti-RNF23 antibody (ab49312) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-RNF23 antibody (ab49312)

ab49312 has not yet been referenced specifically in any publications.

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