Overview

  • Product nameAnti-RNF25 antibody
    See all RNF25 primary antibodies
  • Description
    Mouse monoclonal to RNF25
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment, corresponding to amino acids 352 - 460 of Human RNF25, with a proprietary tag (NP_071898).

  • Positive control
    • Human testis tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab118320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 mg/ml. Predicted molecular weight: 51 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein in western blotting.
IHC-P Use a concentration of 3 µg/ml.

Target

  • FunctionE3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). Stimulates transcription mediated by NF-kappa-B.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 RING-type zinc finger.
    Contains 1 RWD domain.
  • DomainThe RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.
  • Post-translational
    modifications
    Ubiquitinated.
  • Information by UniProt
  • Database links
  • Alternative names
    • AO7 antibody
    • E3 ubiquitin protein ligase RNF25 antibody
    • E3 ubiquitin-protein ligase RNF25 antibody
    • EC 6.3.2.- antibody
    • FLJ13906 antibody
    • RING finger protein 25 antibody
    • RING finger protein AO7 antibody
    • RNF 25 antibody
    • RNF25 antibody
    • RNF25_HUMAN antibody
    see all

Anti-RNF25 antibody images

  • Anti-RNF25 antibody (ab118320) at 1 µg/ml + Recombinant RNF25 protein (the immunogen) with a proprietary tag at 0.2 µg

    Predicted band size : 51 kDa
  • ab118320, at 3 ug/ml, staining RNF25 in formalin-fixed, paraffin-embedded Human testis tissue by Immunohistochemistry

References for Anti-RNF25 antibody (ab118320)

ab118320 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"