Anti-RNF25 antibody (ab89281)
Key features and details
- Mouse polyclonal to RNF25
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RNF25 antibody
See all RNF25 primary antibodies -
Description
Mouse polyclonal to RNF25 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human RNF25. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- HeLa cell lysate. HeLa nuclear extract. RNF25 transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. |
Target
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Function
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). Stimulates transcription mediated by NF-kappa-B. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 RING-type zinc finger.
Contains 1 RWD domain. -
Domain
The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination. -
Post-translational
modificationsUbiquitinated. - Information by UniProt
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Database links
- Entrez Gene: 64320 Human
- SwissProt: Q96BH1 Human
- Unigene: 471403 Human
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Alternative names
- AO7 antibody
- E3 ubiquitin protein ligase RNF25 antibody
- E3 ubiquitin-protein ligase RNF25 antibody
see all
Images
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Anti-RNF25 antibody (ab89281) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Anti-RNF25 antibody (ab89281) at 1 µg/ml + HeLa Nuclear extract at 50 µg
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
All lanes : Anti-RNF25 antibody (ab89281) at 1 µg/ml
Lane 1 : RNF25 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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Datasheet download
References (1)
ab89281 has been referenced in 1 publication.
- Cho JH et al. RNF25 promotes gefitinib resistance in EGFR-mutant NSCLC cells by inducing NF-?B-mediated ERK reactivation. Cell Death Dis 9:587 (2018). PubMed: 29789542